人类肺内皮细胞的综合单细胞地图
Jonas C Schupp1, Taylor S Adams1, Carlos Cosme1
1Pulmonary, Critical Care and Sleep Medicine (J.C.S., T.S.A., C.C., N.O., M.C., K.-A.R., E.P.M., M.S., G.D., F.A., N.N., X.Y., N.K.), Yale University School of Medicine, New Haven, CT.
Circulation
|May 25, 2021
概括
这项研究提供了一个全面的人类肺内皮细胞图谱,揭示了以前未知的细胞类型和跨物种的保存标记. 这种资源增强了对肺血管多样性和疾病状况的理解.
科学领域:
- 肺和血管生物学
- 单细胞基因组学
- 人体解剖学与生理学
背景情况:
- 人类肺内皮的细胞多样性仍然不完全理解.
- 需要对肺内皮细胞 (ECs) 进行系统的表征,以了解它们的表型多样性和组成.
- 现有的知识差距阻碍了对肺血管结构和功能的详细了解.
研究的目的:
- 创建人类肺内皮细胞 (ECs) 的参考地图.
- 为了更深入地了解肺内皮的表型多样性和细胞组成.
- 识别和描述人类肺部内的新型内皮细胞亚群.
主要方法:
- 从六个人类肺部数据集中重新处理的单细胞RNA测序 (scRNAseq) 数据.
- 进行代聚类和差异表达分析以描述EC群体.
- 使用光显微镜和现场杂交验证标记基因;使用小鼠和肺高血压数据分析跨物种和疾病相关性.
主要成果:
- 从73个个体中鉴定出超过15,000个血管EC, 显示出不同的内皮血统特征.
- 发现了以前无法区分的子群体,包括肺静脉 (COL15A1neg) 和全身静脉 (COL15A1pos) EC,以及确认的气球细胞.
- 在小鼠中证实了所有六种EC类型的存在,确定了保存的标记基因,并观察到肺高血压中维持的内皮多样性.
结论:
- 在正常人肺中开发了全面的EC参考图谱.
- 在人类和小鼠中确认并详细介绍以前未被识别的内皮细胞群.
- 这本地图书为研究肺血管生物学和疾病提供了宝贵的资源.
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