一个相隔的核GBPL电路控制植物的免疫力
Shuai Huang1,2,3,4, Shiwei Zhu1,2,3,4, Pradeep Kumar1,2,3,4
1Howard Hughes Medical Institute, New Haven, CT, USA.
Nature
|May 27, 2021
概括
植物酸结合蛋白 (GBP) 类GTPases (GBPLs) 通过液相分离 (LLPS) 形成核凝结物,以抵御生物应激. 这种GBPL电路突显了相分离凝结物的植物免疫作用.
科学领域:
- 细胞生物学
- 植物科学
- 生物化学
背景情况:
- 液相分离 (LLPS) 对于在无膜细胞内组织细胞活动至关重要.
- 植物免疫依赖于复杂的分子机制来检测和应对病原体.
研究的目的:
- 识别和描述涉及植物防御机制的新型蛋白质.
- 阐明LLPS在植物免疫反应中的核分离中的作用.
主要方法:
- 在Arabidopsis thaliana中识别酸盐结合蛋白 (GBP) 类GTPase (GBPLs).
- 在现场冷电子断层扫描可视化核凝聚物.
- 生物化学测定用于研究蛋白质-蛋白质相互作用和转录调节.
主要成果:
- 在生物应激时,GBPL1和GBPL3蛋白质通过核中的LLPS驱动GBPL防御激活凝聚物 (GDAC) 的形成.
- 在基底条件下,GBPL1将GBPL3隔离,但GBPL3LLPS是由免疫线索触发的.
- GDACs将转录协活性剂和RNA聚合酶II招募到防御基因促进剂,重新编程基因表达以抵抗疾病.
结论:
- GBPLs在工厂中形成了一个新的防御电路,利用LLPS创建功能性的核凝结物.
- 这些由GBPL介导的凝聚物对于对病原体进行有效的转录防御反应至关重要.
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