协调血管压素信使RNA的荷尔蒙和突触调节
F Baldino1, T M O'Kane, S Fitzpatrick-McElligott
1Medical Products Department, E. I. du Pont de Nemours & Company, Wilmington, DE 19898.
概括
侧面隔膜核中的单边病变在腎上腺切除术后在大脑中增加了阿尔金血压素 (AVP) mRNA表达. 这突出了成熟的中枢神经系统神经元中AVPmRNA水平的突触影响.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
背景情况:
- 上腺切除术增加了成人副腹腔核中的氨酸血管压素 (AVP) 传递 RNA (mRNA).
- 对于AVP mRNA表达的精确调节机制还没有完全理解.
研究的目的:
- 为了研究侧面隔膜核 (LSN) 病变对AVP mRNA表达在腎上腺切除术后的影响.
- 探索突触影响在调节AVPmRNA水平中的作用.
主要方法:
- 在成年大鼠的侧面隔膜核中产生单侧病变.
- 进行了上腺切除术.
- 在现场杂交被用来量化特定大脑核中的AVPmRNA水平,特别是对心室核 (PVN).
- 免疫组织化学被用来检查AVP和催产素的同位化.
主要成果:
- 单边的LSN病变显著增强了上腺切除术诱导的AVPmRNA水平的增加.
- 这种增强在病变部位的ipsilaterally观察到.
- 这种效应在面向侧腔室核和相关核中最为明显.
- 一个子集的神经元显示了AVP和催产素的局部化,以及它们各自的mRNAs.
结论:
- 来自侧面隔膜核的突触影响调节了阿尔金氨酸血压素mRNA表达.
- 这些发现表明,中枢神经系统通过成熟大脑的突触介导机制动态选择神经元表型.
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