相关实验视频

Updated: Nov 3, 2025

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

分泌的凝抑制了DNGR-1依赖的交叉呈现和癌症免疫力

Evangelos Giampazolias¹, Oliver Schulz¹, Kok Haw Jonathan Lim²

¹Immunobiology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.

|June 3, 2021

概括

通过阻断树突细胞抗原的吸收,分泌的凝 (sGSN) 抑制了抗癌免疫反应. 减少sGSN可以增强抗瘤免疫力和免疫疗法的有效性,从而为癌症治疗提供了新的点.

科学领域:

免疫学
癌症生物学
分子生物学

背景情况:

1型常规树突细胞 (cDC1s) 通过抗原交叉呈现对抗癌CD8+T细胞反应至关重要.
cDC1s利用DNGR-1受体将F-actin与死细胞结合,从而促进抗原交叉呈现.
分泌的凝素 (sGSN) 是一种细胞外蛋白质,在免疫调节中具有潜在的作用.

研究的目的:

研究分泌的凝素 (sGSN) 在调节cDC1s的抗原交叉呈现中的作用.
确定sGSN对抗瘤免疫力和癌症免疫治疗的影响.
在人类癌症中探索sGSN和actin细胞骨相关蛋白的临床相关性.

主要方法:

评估了sGSN对cDC1s对F-actin和抗原交叉呈现的DNGR-1结合的影响.
使用sGsn缺乏的小鼠来评估瘤耐药性和免疫治疗反应.
分析了人类癌症数据集的sGSN水平,行为细胞骨突变,抗癌免疫力和患者存活率之间的相关性.

主要成果:

分泌的凝 (sGSN) 抑制了DNGR-1介导的F-actin结合和cDC1的抗原交叉呈现.
缺乏sGsn的小鼠表现出增强的DNGR-1依赖性对瘤的抵抗力和改善的免疫治疗结果.
抗癌免疫特征和人类癌症中更好的存活率相关的内sGSN和actin细胞骨蛋白的突变.

结论:

通过抑制cDC1介导的抗原交叉呈现,抑制抗瘤CD8+T细胞反应.
向sGSN或调节actin细胞骨相互作用是增强癌症免疫疗法的潜在策略.
这些发现突显出细胞外蛋白,细胞机械和抗瘤免疫之间的复杂相互作用.

关键词:

其他国家 DNGR-1的使用情况 F-actin 的使用情况癌症免疫力交叉呈现树突细胞分泌的凝

更多相关视频

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

Receptor Downregulation in MVBs

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...