编码核因子IRF-1的基因的调节表达,该基因特别与IFN-β基因调节元素结合
M Miyamoto1, T Fujita, Y Kimura
1Institute for Molecular and Cellular Biology, Osaka University, Japan.
Cell
|September 9, 1988
概括
干扰素调节因子-1 (IRF-1) 对病毒诱导的干扰素-β (IFN-β) 基因表达至关重要. 这项研究详细介绍了IRF-1的克隆,并揭示了它在调节其他免疫基因及其自身病毒诱导性促进物的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 干扰素-β (IFN-β) 基因表达是由病毒感染引发的.
- 一种小鼠核因子,干扰素调节因子-1 (IRF-1),与人类的IFN-β基因促进体结合,并调解病毒诱导.
- 在免疫反应中IRF-1的确切作用和调节需要进一步阐明.
研究的目的:
- 为了分子克隆和表征鼠标和人类编码IRF-1的cDNA.
- 研究IRF-1在调节其他免疫相关基因中的作用.
- 通过病毒和非病毒刺激来探索IRF-1基因表达的诱导性.
主要方法:
- 克隆和测序小鼠和人类IRF-1的cDNA.
- 对IFN-β促进体的IRF-1结合的分析.
- 对IRF-1,IFN-alpha和MHCI类基因的基因表达分析.
- 在细胞系中使用纽卡斯尔病病毒和康卡纳瓦林A的诱导研究.
主要成果:
- 编码小鼠和人类IRF-1的cDNA被成功克隆和特征化.
- 似乎IRF-1可以调节IFN-alpha和MHCI类基因的表达.
- IRF-1基因表达在L929细胞中的纽卡斯尔病病毒和脏细胞中的康卡纳瓦林A显著诱导.
- IRF-1基因促进体表现出病毒诱导活性.
结论:
- IRF-1 是一个关键的转录因子,参与了对病毒感染的先天免疫反应.
- 在IFN-β之外,IRF-1在调节多个免疫基因方面发挥着作用.
- IRF-1的表达本身受到病毒和线粒体刺激的强烈诱导,突出了调控反循环.
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