Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

19.7K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
19.7K
The Nucleosome01:19

The Nucleosome

3.1K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
3.1K
Chromatin Packaging01:32

Chromatin Packaging

18.0K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
18.0K
Chromatin Packaging02:21

Chromatin Packaging

18.2K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
18.2K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

50.3K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
50.3K
DNA Packaging00:58

DNA Packaging

108.8K
Overview
108.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Thalassaemia.

Nature reviews. Disease primers·2026
Same author

CDIN1-Codanin-1 complex defective in congenital dyserythropoietic anaemia type I is an RNA nuclease.

Nature communications·2026
Same author

ChatMDV: Reducing Technical Barriers in Bioinformatics Analysis using Large Language Models.

GigaScience·2026
Same author

The fetal specific gene LIN28B is essential for human fetal B-lymphopoiesis and initiation of KMT2A::AFF1 infant leukemia.

Blood·2026
Same author

Loop extrusion by cohesin plays a role in enhancer-activated gene expression early in differentiation.

Nature communications·2026
Same author

Mezigdomide reverses T-cell exhaustion through degradation of IKZF1/IKZF3 and reinvigoration of cytokine production pathways.

Blood·2026

相关实验视频

Updated: Nov 2, 2025

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.8K

在基因对分辨率下定义基因组架构

Peng Hua1, Mohsin Badat1, Lars L P Hanssen1

  • 1MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.

Nature
|June 10, 2021
PubMed
概括

研究人员开发了Micro-Capture来绘制基因调节接触的基数对分辨率. 这种方法揭示了转录因子和染色质环挤出如何维持组织特异性基因表达的增强剂-促进剂相互作用.

更多相关视频

Mapping Mammalian 3D Genome Interactions with Micro-C-XL
11:41

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

3.0K
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

410.2K

相关实验视频

Last Updated: Nov 2, 2025

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.8K
Mapping Mammalian 3D Genome Interactions with Micro-C-XL
11:41

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

3.0K
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

410.2K

科学领域:

  • 基因组学
  • 分子生物学
  • 表观遗传学

背景情况:

  • 在真核生物中,基因表达受到增强剂的调节,它们往往远离促进剂.
  • 增强剂和促进剂之间的物理接触对基因调节至关重要.
  • 之前的方法缺乏在蛋白质水平上绘制这些接触的分辨率.

研究的目的:

  • 开发一种高分辨率的方法来绘制基因调节元件之间的物理联系.
  • 研究转录因子和染色质结构在维持增强剂-促进剂相互作用中的作用.

主要方法:

  • 开发了微捕获-C,一种染色体形状捕获技术.
  • 实现基对分辨率以映射监管元素之间的相互作用.
  • 分析了增强剂,促进剂和CCCTC结合因子 (CTCF) 位点之间的接触.

主要成果:

  • 在增强剂,促进剂和CTCF站点之间确定了高度特定的接触.
  • 证明了转录因子在维持增强剂-促进剂接触中的关键作用.
  • 观察到与干预色素中的活性促进剂和增强剂相关的CTCF位相互作用增加.

结论:

  • 微捕获-C为研究色素相互作用提供了前所未有的分辨率.
  • 转录因子是维持增强剂-促进剂循环的关键因素.
  • 染色质环挤出,依赖于活性调节元件的凝聚荷,解释了组织特异性域的形成.