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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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相关实验视频

Updated: Nov 2, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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使用Seq-Scope对空间转录组进行显微镜检查

Chun-Seok Cho1, Jingyue Xi2, Yichen Si2

  • 1Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Cell
|June 11, 2021
PubMed
概括
此摘要是机器生成的。

Seq-Scope是一种新的空间条形码技术,可以实现光学显微镜分辨率的转录形状. 这一突破使单细胞和亚细胞层面的组织空间转录组异质性的详细可视化成为可能.

关键词:
捕获RNA的方法空间转录学大结肠高分辨率组织学肝脏分子条形码scRNA-seq 在空间单细胞分析亚细胞分析

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科学领域:

  • 分子生物学
  • 基因组学
  • 生物技术

背景情况:

  • 目前的空间条形码技术提供了有限的分辨率,阻碍了对转录形状的详细组织学分析.
  • 高分辨率的空间转录学对于理解组织结构和细胞异质性至关重要.

研究的目的:

  • 推出Seq-Scope,一种具有可比光学显微镜分辨率的新型空间条形码技术.
  • 展示Seq-Scope在各种尺度上可视化空间转录组异质性的能力.

主要方法:

  • Seq-Scope在Illumina测序平台上使用随机编码的单分子寡核酸的固相放大.
  • 空间坐标被分配给条码集群,然后处理以暴露RNA捕获部分.
  • 这创建了大约0.50.8μm的像素,定义了该技术的高分辨率.

主要成果:

  • Seq-Scope可视化跨多种组织学尺度的空间转录组异质性,包括组织区分 (例如肝脏,结肠) 和炎症纤维化轴.
  • 该技术解决了细胞组件,包括单细胞类型和亚型,以及细胞核和细胞质等亚细胞结构.
  • 在组织段内展示了RNA分布的高分辨率映射.

结论:

  • Seq-Scope通过提供前所未有的分辨率, 显著推进了空间转录组分析.
  • 这项技术快速,简单,精确,易于使用,为生物医学研究人员提供空间单细胞分析.
  • 使组织组织和细胞相互作用能够在细微的尺度上进行详细的调查.