植物的"辅助"免疫受体是Ca2+透的非选择性阴离子通道
Pierre Jacob1, Nak Hyun Kim1, Feihua Wu2,3
1Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
概括
植物免疫受体,辅助NLRs,形成通道来控制细胞死亡. 这一发现揭示了这些关键蛋白质如何直接调节植物防御信号通路.
科学领域:
- 植物免疫力 植物免疫力
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 植物核酸结合氨酸丰富的重复受体 (NLRs) 是植物免疫和编程细胞死亡的关键调节者.
- 一个子家族的
- 一个助手,一个助手.
- NLRs作为许多系统的功能必不可少的组件.
- 传感器 传感器 传感器
- 植物防御中的NLRs.
研究的目的:
- 研究辅助NLRs,特别是NRG1.1和ADR1调节植物免疫和细胞死亡的分子机制.
- 为了确定辅助NLR是否直接形成参与信号传递的离子通道.
主要方法:
- 生物化学试验研究NRG1.1的寡合化和局部化.
- 在植物和人类细胞中进行功能分析,以评估离子 (Ca2+) 流入.
- 电生理学记录 (全电池电压) 用于描述离子通道活动.
- 使用影响寡合化,局部化和蛋白质充电的突变物进行遗传分析.
主要成果:
- 发现活跃的NRG1.1有氧化并局部化到血点,在植物和人类细胞中介于Ca2+的流入.
- 依赖NRG1.1的Ca2+流入和细胞死亡对Ca2+通道阻断剂敏感,并因影响寡合化或膜向的突变而受损.
- 包括NRG1.1和ADR1在内的辅助NLR被证明形成Ca2+透性阴离子通道,直接调节细胞质Ca2+水平和细胞死亡.
结论:
- 辅助NLRs作为直接的Ca2+透性阴离子通道起作用.
- 这些通道对于转导植物免疫中的细胞死亡信号至关重要.
- 负电荷的N端残留物对辅助NLRs的Ca2+流入和细胞死亡功能至关重要.
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