相关实验视频

Updated: May 10, 2026

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

小鼠大脑皮层细胞多样化的分子逻辑

Daniela J Di Bella^1,2, Ehsan Habibi^1,3, Robert R Stickels²

¹Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.

|June 24, 2021

概括

研究人员使用单细胞测序绘制了老鼠新皮质的发展图, 以了解多样化的脑细胞类型的形成. 这本地图书揭示了指导细胞发育的基因调控程序,并确定了发育异常的起源.

科学领域:

神经科学
发育生物学
基因组学

背景情况:

哺乳动物的大脑皮层表现出显著的细胞类型多样性,
在发育过程中驱动皮质细胞类型的分子机制的理解是有限的.
动态细胞状态转换和延长的发育时间表使分析复杂化.

研究的目的:

创建一个关于小鼠新皮层发育的综合图谱.
阐明控制皮层细胞类型建立和组织的分子逻辑.
确定控制细胞谱系决策和分化的基因调控程序.

主要方法:

采用单细胞RNA测序 (scRNA-seq) 和使用测序 (scATAC-seq) 的单细胞染色体检测.
在胚胎发育和出生后早期每天采集新皮层样本.
综合空间转录和计算轨迹重建.

主要成果:

创建了新皮层发育的高分辨率地图,捕捉了细胞状态的过渡.
重建了各种皮层细胞类的发育轨迹.
推断的空间组织和基因调控网络控制细胞多样化和血统分支.

结论:

这项研究提供了推动新皮层细胞多样化的监管机制的全球视角.
发育地图有助于精确地确定血统特异性的发育异常的起源.
这种资源有助于了解皮质发生和相关疾病.

更多相关视频

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

相关概念视频

Diversity in Cell Signaling Responses

Diversity in Cell Signaling Responses

The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...