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相关概念视频

The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

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Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
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Rab Cascades01:25

Rab Cascades

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Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Structure of Cadherins01:25

Structure of Cadherins

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The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
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Catenins01:23

Catenins

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Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
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Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

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The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
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Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

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Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
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相关实验视频

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Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
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在模型系统中,表达的重组阴蛋白调解细胞分类.

A Nose1, A Nagafuchi, M Takeichi

  • 1Department of Biophysics, Faculty of Science, Kyoto University, Japan.

Cell
|September 23, 1988
PubMed
概括

卡德林调解细胞粘附. 在L细胞中表达特定的阴素,如E-阴素或P-阴素,指导细胞分类和组织组织,证明阴素.

科学领域:

  • 细胞生物学 细胞生物学
  • 发展生物学 发展生物学
  • 分子生物学分子生物学

背景情况:

  • 卡德林是关键的细胞表面糖蛋白,调解依赖的细胞对细胞粘附.
  • 假设卡德林的差异表达在组织形态发生和细胞分类中起作用.
  • 缺乏内源性阴素活性的L细胞作为研究阴素功能的模型系统.

研究的目的:

  • 调查特定的阴素子类,E-阴素和P-阴素在调解细胞粘附和分类中的作用.
  • 为了确定卡德林表达是否影响异质群体内的细胞协会.
  • 在发育背景下提供卡德林介导细胞分类的直接证据.

主要方法:

  • 用E-cadherin或P-cadherin表达载体感染L细胞.
  • 评估转染L细胞的细胞聚合和同型结合.
  • 使用转染的L细胞和胚胎肺组织扩展剂进行复制试验.

主要成果:

  • 用E-cadherin或P-cadherin转染的L细胞表现出Ca2+依赖的细胞聚合.
  • 被转染的细胞表现出对表达相同cadherin亚类的其他细胞的偏好粘附.
  • 在复制的胚胎肺组织中,表达E-cadherin的L细胞与上皮结构局部化,而未转移的L细胞与介质细胞组成部分相关.

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结论:

  • 表达的卡德林类型决定了细胞粘附特异性.
  • 差异性卡德林表达是推动复杂细胞环境中的细胞分类的关键机制.
  • 这些发现直接证明了卡德林在组织组织和组织发育中的作用.