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相关概念视频

RNA Splicing01:32

RNA Splicing

58.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
58.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

22.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
22.7K
Alternative RNA Splicing02:18

Alternative RNA Splicing

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4.3K
Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.6K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.6K
What is Gene Expression?01:36

What is Gene Expression?

9.9K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
9.9K

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相关实验视频

Updated: Nov 1, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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药理学调节RNA拼接增强抗瘤免疫力

Sydney X Lu1, Emma De Neef2, James D Thomas3

  • 1Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA; Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA.

Cell
|June 25, 2021
PubMed
概括
此摘要是机器生成的。

药理拼接调节产生癌症新抗原,增强抗瘤免疫力和检查点免疫疗法. 这种方法提供了一种通过引起T细胞反应改善癌症治疗的新策略.

关键词:
其他其他类型一个RBM39核糖核酸拼接免疫检查点封锁免疫体免疫疗法新抗原新生态接合方式

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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相关实验视频

Last Updated: Nov 1, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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科学领域:

  • 癌症学
  • 免疫学
  • 分子生物学

背景情况:

  • 已知DNA突变是影响免疫检查点阻断反应的新抗原来源.
  • 癌细胞中RNA剪接的变化也可以产生新表位,但它们的抗原性和临床潜力在很大程度上仍未被探索.

研究的目的:

  • 调查药理拼接调节是否可以产生新抗原.
  • 确定拼接调节是否可以引起抗瘤免疫力并增强检查点免疫疗法.

主要方法:

  • 使用特定药物类别对RNA拼接进行药理学调制.
  • 在体内评估瘤生长抑制和抗瘤免疫力.
  • 对MHC类I的T细胞依赖性和呈现的分析.
  • 拼接变化和免疫体的特征.

主要成果:

  • 拼接调节产生了真正的新抗原并引起了抗瘤免疫力.
  • 这种方法抑制了瘤的生长并增强了检查点阻塞的有效性.
  • 这些效应取决于瘤MHC类I的宿主T细胞和.
  • 跨瘤类型的刻板结合变化改变了MHC I结合的免疫体,产生了免疫结合衍生的新体.

结论:

  • 拼接调节是免疫性的未开发来源.
  • 这种策略可以增强对免疫检查点封锁的反应.
  • 拼接调节是改善癌症免疫疗法的临床可用方法.