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促进体反意义RNA的形状调节了联体诱导的转录激活
Fan Yang1, Bogdan Tanasa2, Rudi Micheletti3
1Howard Hughes Medical Institute, Department and School of Medicine, University of California, San Diego, La Jolla, CA, USA. fay009@health.ucsd.edu.
Nature
|July 1, 2021
概括
长非编码RNAs (lncRNAs),特别是促进器反意义RNAs (PAS),通过控制P-TEFb复合体来调节基因转录. 通过结构相互作用,PAS RNAs招募脱甲基酶,导致基因激活.
科学领域:
- 基因组学
- 分子生物学
- 核糖核酸生物学
背景情况:
- 长非编码RNAs (lncRNAs) 在哺乳动物基因组调节中起着至关重要的作用.
- 促销器反意义RNA (PAS) 是一种具有辩论功能的特定类 lncRNA.
- 了解RNA的全基因组分布和功能至关重要.
研究的目的:
- 开发一种用于定量检测基因组中的RNA分布的新方法.
- 阐明PASRNAs在转录调节中的作用.
- 发现PASRNA控制基因表达的机制.
主要方法:
- 通过RNA-Cas13a复合体 (CIRC) 测定进行染色体分离的发展.
- 在基因组中定量检测RNA分布.
- 对H3K9me3,HP1α和KAP1基因促进体的招募和释放的分析.
主要成果:
- 通过关闭7SK小核RNA-P-TEFb复合体,PAS RNAs作为转录暂停释放的守门人.
- 激活ERα诱导PASRNAs,导致H3K9me3的丧失和HP1α和KAP1的释放.
- 通过RNA干环结构调解的H3K9me3脱甲酶的PASRNA依赖性招募对于基因激活至关重要.
结论:
- 通过结构特征,PAS RNAs招募脱甲基酶来调节基因转录.
- 涉及化KAP1的ERα-MegaTrans增强器途径促进17β-雌激素诱导的基因激活.
- 这项研究揭示了基于RNA结构的PASRNA介导基因调节机制.
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