概括
研究人员在E. coli中的核糖体RNA (rRNA) 操作子末端确定了特定的转移RNA (tRNA) 基因. 这些远端tRNA基因需要rRNA促进体进行表达,这表明与rRNA基因的共同转录.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 微生物学 微生物学
背景情况:
- 之前已经分离了七个rRNA操作子,并确定了tRNA基因.
- 描述了16S和23SrRNA基因之间的间隔tRNA基因安排.
- 在rRNA操作子的远端或附近发现了各种tRNA基因.
研究的目的:
- 在rRNA操作子中研究远端tRNA基因的表达和定位.
- 确定rRNA促进体在远端tRNA基因表达中的作用.
- 确认远端tRNA基因与rRNA基因的共同转录.
主要方法:
- 对缺乏rRNA促进体的混合质粒进行分析.
- 利芬素运行试验,以评估基因与促进者的接近性.
- 遗传映射和表达研究.
主要成果:
- 远端tRNA基因的表达过程中,rRNA促进体是必不可少的.
- 该tRNATrp基因位于远离促销器的距离远于间距tRNA或5SRNA基因.
- 对于tRNATrp和tRNAAsp1的基因在rrnC操作子的远端得到确认.
结论:
- 远端tRNA基因,包括 rrnC操作子上的tRNATrp和tRNAAsp1,与rRNA基因共同转录.
- 该rRNA促进体驱动这些远端tRNA基因的表达.
- 建议其他已识别的远端tRNA也是rRNA操作子的组成部分.
相关概念视频
Operons
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
Operons
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Transcription Attenuation in Prokaryotes
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
Repressible Operon: trp Operon
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...


