相关实验视频

Updated: Oct 29, 2025

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

Published on: January 5, 2016

用酸乙醇胺对T_FH细胞和体内免疫的代谢控制

Guotong Fu¹, Clifford S Guy¹, Nicole M Chapman¹

¹Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA.

|July 8, 2021

概括

通过控制脂乙醇胺 (PE) 水平,该CDP-乙醇胺途径调节T毛囊辅助细胞 (T_FH) 的分化和幽默免疫力,从而稳定T_FH细胞上的CXCR5表达.

科学领域:

免疫学
细胞生物学
代谢途径

背景情况:

毛囊T辅助细胞 (T_FH) 对于B细胞介导的幽默免疫是必不可少的.
虽然BCL6等转录因子是T_FH细胞分化的已知驱动因素,但转录后和代谢程序的作用在很大程度上仍未被探索.

研究的目的:

研究细胞二酸 (CDP) - 乙醇胺途径在T_FH细胞编程和幽默免疫中的作用.
在该途径中识别调节T_FH细胞分化和功能的特定酶.

主要方法:

在小鼠体内进行CRISPR-Cas9查.
确定基因的功能验证.
分析T细胞中的脂质代谢和蛋白质表达.

主要成果:

涉及ETNK1,PCYT2和SELENOI酶的CDP-乙醇胺通路可以选择性地调节T_FH细胞分化.
这种途径通过控制酸丁乙醇胺 (PE) 水平来促进CXCR5的表达和功能,从而防止CXCR5的内部化.
在T细胞中PCYT2的遗传删除会损害T_FH分化和幽默免疫反应.

结论:

脂代谢,特别是CDP-乙醇胺通路,在调节T_FH细胞分化后起着至关重要的作用.
这一途径突出了对免疫信号和效应器程序的新奇代谢控制机制,影响了 humoral 免疫力.

更多相关视频

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Published on: November 21, 2018

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Published on: October 19, 2021

相关概念视频

Synthesis of Phosphatidylcholine in the ER Membrane

Synthesis of Phosphatidylcholine in the ER Membrane

The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...