这种SARS-CoV-2 RBD抗体可以最大限度地扩展和抵抗逃生
Tyler N Starr1, Nadine Czudnochowski2, Zhuoming Liu3
1Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Nature
|July 14, 2021
概括
研究人员发现了针对SARS-CoV-2的强有力的治疗抗体,这些抗体可以抵抗病毒逃逸并中和多种类型的沙贝科病毒. 这些发现为当前和未来的流行病开发有效的抗体疗法提供了指导.
科学领域:
- 病毒学
- 免疫学
- 治疗抗体的开发
背景情况:
- 理想的SARS-CoV-2抗体需要对病毒逃逸的抵抗力,对沙贝科病毒的广泛活性,以及强大的中和和效应功能.
- 了解抗体特性中的表位特异性对于开发有效疗法和为疫苗设计提供信息至关重要.
研究的目的:
- 综合描述针对受体结合域 (RBD) 的SARS-CoV-2抗体的逃逸,范围和强度.
- 确定针对当前和未来的冠状病毒流行病的治疗特征和抗体特征.
主要方法:
- 针对RBD的SARS-CoV-2抗体组的表征.
- 评估体外中和效能,沙贝科病毒的结合宽度和对病毒逃逸的抵抗力.
- 在子模型中评估抗体亲和力,表位素向 (包括神秘表位素和ACE2受体结合动机/RBM),以及体内保护功效.
主要成果:
- 在体外中和化功效和沙贝科病毒结合宽度之间存在权衡.
- 鉴定出具有异常宽度和抗 SARS-CoV-2 逃逸的中和抗体,例如 S2H97,该抗体针对的是一个神秘的表位.
- 具有强大的RBM向抗体 (S2E12),具有广泛的沙贝科病毒活性和高病毒逃逸障碍.
结论:
- 在不同的RBD表位上,抗体逃逸,宽度和强度有显著差异.
- 针对神秘表位或特定RBM区域的抗体显示出治疗开发的有希望的特征.
- 这些发现为疫情准备优先考虑抗体目标和特征提供了关键的见解.
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