光检测的中红外光热显微镜
Minghe Li1, Aleksandr Razumtcev1, Ruochen Yang2
1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.
Journal of the American Chemical Society
|July 16, 2021
概括
我们开发了一种新的显微镜技术,即光检测光热红外 (F-PTIR) 显微镜,用于分析无形固体分散 (ASD). 在暴露在水中的自闭症患者中,F-PTIR揭示了富含药物的域,这对于了解药物的稳定性和溶解至关重要.
科学领域:
- 谱学和显微学
- 材料科学
- 制药科学
背景情况:
- 无形固体分散 (ASD) 对于提高药物的溶解性和生物可用性至关重要.
- 在自闭症患者中,相分离可以显著影响药物溶解动力学和稳定性.
- 在不均的ASD中描述局部化学成分仍然具有挑战性.
研究的目的:
- 引入和验证光检测光热红外显微镜 (F-PTIR) 用于分析ASD.
- 证明F-PTIR在ASD中无标签化学特征的能力.
- 研究水吸附对模型ASD相位行为的影响.
主要方法:
- 开发用于光检测光热红外显微镜 (F-PTIR) 的仪器.
- 使用温度依赖的光量子效率变化来检测局部红外吸收.
- 将F-PTIR应用于模型系统 (聚乙烯糖醇,凝) 和里托纳维尔/聚乙烯基烯酸 (PVPVA) ASD.
主要成果:
- F-PTIR显微镜提供高空间分辨率的红外光谱分析,由光成像指导.
- 使用基和蛋白质微粒的内在自发光实现了无标签的F-PTIR.
- 不同质的瑞托纳维尔/ PVPVA ASD 的表征显示在水吸附时形成富含药物域,支持相分离.
结论:
- F-PTIR显微镜是一种强大的工具,用于在自闭症患者中无标记的微观成分的化学表征.
- 在里托纳维尔/ PVPVA ASD 中,水吸附会诱导相分离,从而产生富含药物的域.
- 了解自闭症阶段分离对于优化药物输送和稳定性至关重要.
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