蜂诺丁-2和诺丁:带有阿斯巴提胺后翻译修饰的拉索
Li Cao1, Moshe Beiser2, Joseph D Koos3
1Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States.
Journal of the American Chemical Society
|July 20, 2021
概括
蛋白质l-异甲基转移酶 (PIMT) 同类物通过形成刚性化阿斯巴提胺环来修改拉索. 这一发现揭示了PIMTs在拉索生物合成中的新后翻译修饰 (PTM) 功能.
科学领域:
- 生物化学
- 分子生物学
- 结构生物学
背景情况:
- 拉索是一种由核糖体合成和翻译后修饰的 (RiPPs),其特点是具有独特的线程结构和异键.
- 已经确定了其他翻译后修饰 (PTM),进一步多样化了拉索的结构和功能.
研究的目的:
- 研究与拉索生物合成基因集群 (BGCs) 相关的蛋白质l-异甲基转移酶 (PIMT) 同类的新型作用.
- 描述PIMT介导的变化对激光的机制和结构影响.
主要方法:
- 基因组挖掘以识别与PIMT同类同位的拉索BGC.
- 修饰的拉索 (cellulonodin-2和lihuanodin) 的异构表达和结构特征.
- 在实验室中制PIMT介导的阿斯巴提胺形成.
主要成果:
- 鉴定了与拉索BGC相关的PIMT同类物,表明一种新的PTM途径.
- 证明这些PIMT在拉索环内甲基化酸侧链,形成稳定的阿斯巴提胺.
- 与未经修改的同类药物相比,阿斯巴提胺修改的拉索具有更高的刚性.
- 观察到与拉索相关的PIMTs的快速甲基转移酶活性,特定于拉索基质.
结论:
- 蛋白质l-异甲基转移酶 (PIMTs) 在翻译后修改拉索中具有新功能,与它们已知的蛋白质修复作用不同.
- 在拉索环内由PIMT介导的阿斯巴提胺部分的形成增强了结构刚性.
- 这种PTM途径在多种生物中保持,突出显示了它在拉索多样化中的重要性.
更多相关视频
12:02An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity
Published on: November 2, 2016
12.3K
11:09Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
Published on: August 1, 2018
10.9K
相关概念视频
01:26Protein Modifications in the RER
6.1K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
6.1K
02:04Covalently Linked Protein Regulators
8.1K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
8.1K