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植物PIEZO同类物在尖端生长过程中调节真空细胞形态

Ivan Radin¹, Ryan A Richardson¹, Joshua H Coomey¹

¹Department of Biology and NSF Center for Engineering Mechanobiology, Washington University in St. Louis, St. Louis, MO, USA.

Science (New York, N.Y.)

|July 30, 2021

概括

植物PIEZO通道对于细胞生长和机械感知至关重要. 和Arabidopsis PIEZO的蛋白质定位在真空膜上,影响真空膜结构和细胞动态.

科学领域:

植物生物学植物生物学
细胞生物学细胞生物学
生物物理学的生物物理.

背景情况:

皮埃索蛋白是动物中发现的必不可少的机械感应离子通道.
它们在植物细胞中的功能,特别是在尖端生长的细胞中,仍然在很大程度上未被探索.
机械感知对于植物发育至关重要,它影响细胞生长和对刺激的反应等过程.

研究的目的:

为了研究PIEZO同类在的尖端生长细胞Physcomitrium patens和花植物Arabidopsis thaliana中的作用.
为了确定植物细胞内PIEZO蛋白的局部和功能.
阐明PIEZO通道对细胞过程 (如生长,信号传递和真空管动力学) 的贡献.

主要方法:

在Physcomitrium patens和Arabidopsis thaliana中对PIEZO功能进行比较分析.
对PIEZO同类的功能丧失,功能获取和过度表达突变的生成和分析.
使用显微镜确定PIEZO蛋白质位置的亚细胞局部化研究.
研究细胞表型,包括细胞生长,大小,细胞质振荡和真空细胞形态.

主要成果:

在PIEZO1和PIEZO2中,Pp1和Pp2在PIEZO2中冗余调节生长,大小和振荡. 病原体阴毛细胞.
这两种PIEZO同类物都局部存在于真空膜 (tonoplast).
摩斯PIEZO蛋白质通过管道,内化和裂变促进真空膜复杂性.
PIEZO1也定位在质体上,对于花粉管尖的真空管道是必不可少的.

结论:

植物PIEZO同类物参与机械传感过程和细胞生长.
质体作为植物PIEZO蛋白的关键局部化部位,与动物的血膜局部化不同.
通过PIEZO通道调节的真空膜动力学对于尖端生长的植物细胞至关重要.
质体的移动性可能有助于植物细胞中的机械感知功能.

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