与ATP的弱非特异性相互作用对蛋白质的影响
Mayu Nishizawa1, Erik Walinda2, Daichi Morimoto1
1Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Journal of the American Chemical Society
|August 2, 2021
概括
腺三酸盐 (ATP) 作为一种水,与蛋白质相互作用很弱. 这种代谢物与蛋白质的相互作用会改变蛋白质的水分,并可能在细胞中提供一般的表面保护.
科学领域:
- 生物化学和生物物理
- 分子生物学
- 蛋白质聚合
背景情况:
- 氨酸三酸 (ATP) 是一种重要的代谢物,在能量转移,核酸合成和信号传递中发挥着重要作用.
- 最近的假设表明,ATP作为一种水,可能会抑制氨基原蛋白聚合.
- 精确的机制和ATP对蛋白质行为的一般物理化学效应在很大程度上是未知的.
研究的目的:
- 研究ATP与蛋白质之间的非共价相互作用.
- 为了阐明ATP蛋白共存的物理化学后果.
- 要确定ATP与蛋白质的相互作用是特定的还是一般的.
主要方法:
- 核磁共振 (NMR) 光谱学
- 分子动力学 (MD) 模拟
- 解决方案NMR
- 补充生物物理方法
主要成果:
- 在ATP和各种蛋白质之间观察到弱,度依赖的非共价相互作用,包括内在无序和折叠的蛋白质.
- ATP与蛋白质的相互作用改变了暴露在溶剂中的蛋白质残留物的水分.
- 显示了对测试蛋白质的一般性,非特异性结合.
- 在生理度 (5-10mM) 上,ATP通过依赖和独立途径形成寡合状态.
结论:
- ATP与一系列蛋白质进行弱,一般,非特异的相互作用.
- 这些相互作用可以改变蛋白质的水分,并可能对蛋白质表面产生保护作用.
- 高细胞内ATP度表明与蛋白质的频繁短暂相互作用,有助于代谢物介导的蛋白质表面保护.
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