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MIC-Drop:一个大型体内CRISPR查平台

Saba Parvez¹, Chelsea Herdman², Manu Beerens³

¹Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, UT, USA.

Science (New York, N.Y.)

|August 20, 2021

概括

多重混合的CRISPR滴 (MIC-Drop) 能够在斑马鱼中进行大规模的遗传查. 这种新平台能有效地识别与特定特征和潜在药物点相关的基因.

科学领域:

遗传学
发育生物学
生物工程

背景情况:

由于可扩展性问题,CRISPR-Cas9屏幕在细胞培养中具有强大作用,但在动物模型中具有挑战性.
为了进行大规模的基因查, 制造和追踪大量突变动物目前是无法实现的.
需要有效的,可扩展的方法来对整个生物进行功能性基因选.

研究的目的:

在斑马鱼中开发一个可扩展的CRISPR屏幕平台.
能够识别与形态和行为表型相关的基因.
促进参与生物过程和药物标的新基因的发现.

主要方法:

开发了多重混合的CRISPR滴 (MIC-Drop) 平台.
结合滴滴微流体,大量注入CRISPR核蛋白和DNA条形码.
在斑马鱼的功能性遗传查中应用MIC-Drop.

主要成果:

MIC-Drop成功地在斑马鱼中启用了大规模的CRISPR屏幕.
该平台有效地识别了负责特定形态和行为表型的基因.
在识别小分子点和发现心脏发育中的新基因方面展示了MIC-Drop的实用性.

结论:

MIC-Drop是一个可扩展的基因组规模逆转基因选平台,
这项技术克服了以往动物基CRISPR选的局限性.
MIC-Drop为了解基因功能和确定治疗点开辟了新的途径.

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相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...