Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

5.1K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.1K
RNA Editing02:23

RNA Editing

9.3K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.3K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

3.0K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.0K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.6K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.6K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

3.9K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.9K
Negative Regulator Molecules01:23

Negative Regulator Molecules

36.7K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spaceflight multi-omics reveals vulnerabilities of human germ cell development.

Science advances·2026
Same author

Closed-loop motor imagery brain-computer interface-assisted training for upper limb rehabilitation after subacute stroke: clinical and electroencephalographic outcomes from a randomized pilot trial.

Frontiers in neurology·2026
Same author

Management of Engraftment Arrhythmias Associated with Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Transplantation.

Heart rhythm·2026
Same author

Structural and dynamic insights into the allosteric activation of p38α MAP kinase via specific docking interactions.

Communications biology·2026
Same author

Resveratrol Promotes Goat Myoblast Differentiation via PROX1-Mediated Inhibition of NOTCH Signaling.

The Journal of nutrition·2026
Same author

Effects of motor imagery brain-computer interface task on quantitative EEG features in patients with prolonged disorders of consciousness.

Frontiers in neuroscience·2026

相关实验视频

Updated: Oct 22, 2025

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors
11:49

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Published on: June 23, 2023

904

编辑RNA限制了过度活跃的毛激酶

Dongdong Li1,2,3,4, Yufan Liu1,2,3,4, Peishan Yi1,2,3,4

  • 1Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.

Science (New York, N.Y.)
|August 27, 2021
PubMed
概括

细胞通过RNA编辑来调节高活性蛋白激酶. 在ADR-2中发生的突变,一种RNA腺酶,拯救了由过度活跃的激酶引起的状细胞缺陷,揭示了一个新的反机制.

更多相关视频

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.9K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.1K

相关实验视频

Last Updated: Oct 22, 2025

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors
11:49

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Published on: June 23, 2023

904
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.9K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.1K

科学领域:

  • 分子生物学
  • 遗传学
  • 细胞生物学

背景情况:

  • 精确调节蛋白激酶活动对于细胞功能至关重要.
  • 失调的激酶活性,特别是过度活性,会导致各种细胞缺陷.
  • 控制过活性激酶的机制尚未完全理解.

研究的目的:

  • 调查控制超活性蛋白激酶的调节机制.
  • 确定可以抑制与构成性活性激酶相关的表型的遗传因素.
  • 阐明RNA编辑在酶调节中的作用.

主要方法:

  • 在*C. elegans*中产生构成性活性的基激酶DYF-5 (DYF-5CA).
  • 使用基因抑制屏幕来识别拯救突变.
  • 分析受影响动物的RNA转录,RNA编辑,mRNA拼接和蛋白质翻译.

主要成果:

  • 由DYF-5CA引起的ADR-2 (RNA腺酶) 突变挽救了状细胞缺陷.
  • DYF-5CA诱导反意义RNA的异常转录,形成与DYF-5CAmRNA的双链RNA.
  • ADR-2 异位编辑了 DYF-5CA mRNA,损害了拼接,阻断了翻译,并触发了 mRNA 衰变.
  • 这种依赖RNA编辑的反调节取决于激酶过活性,也观察到其他纤维激酶 (NEKL-4/NEK10和DYF-18/CCRK).

结论:

  • 过度活跃的激酶可以通过依赖RNA编辑的反机制来调节.
  • 通过编辑mRNA,ADR-2在抑制激酶过活方面发挥着关键作用.
  • 这种涉及RNA编辑的调节途径代表了控制纤毛激酶的广泛机制.