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人体肠道的细胞在空间和时间上的映射

Rasa Elmentaite¹, Natsuhiko Kumasaka¹, Kenny Roberts¹

¹Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK.

|September 9, 2021

概括

这项研究使用单细胞RNA测序绘制了人类肠道细胞图,揭示了不同的BEST4上皮细胞和细胞功能. 它提供了关于肠道发育,神经群体和炎症性肠道疾病的见解.

科学领域:

胃肠病学
发育生物学
免疫学

背景情况:

人体肠道的细胞组成是动态的,从发育到成年都会发生变化.
了解这些细胞变化对于理解肠道功能和疾病至关重要.

研究的目的:

综合地绘制人类肠道和成人的细胞系.
在肠道中识别新细胞类型和功能.
研究肠道发育,平衡和炎症性肠病的细胞基础.

主要方法:

单细胞RNA测序和近50万细胞的抗原受体分析.
在发育,儿科和成年人肠道的多个解剖区域进行分析.
系统生物学方法识别关键的细胞驱动因素.

主要成果:

在人类肠道中发现了截然不同的BEST4上皮细胞.
作为肠细胞功能的IgG传感的含义.
在发育中的肠道神经系统中神经细胞群的表征和与赫斯普朗格疾病相关的基因表达的预测.
确定二次淋巴组织形成中的细胞参与者及其在炎症性肠病中的作用.

结论:

这种全面的细胞目录提供了对人类肠道发育,平衡和疾病的新见解.
这些发现突显了肠道细胞程序的可塑性,
这项资源将促进有关肠道疾病和治疗策略的研究.

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