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Updated: Oct 19, 2025

An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

Published on: October 13, 2021

通过系统的时间间隔成像揭示了嗅觉电路组合的细胞基

Tongchao Li¹, Tian-Ming Fu², Kenneth Kin Lam Wong¹

¹Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

|September 22, 2021

概括

这项研究研究了Drosophila嗅觉系统,揭示了嗅觉受体神经元 (ORN) 轴突如何精确地准特定的大脑区域. 这些发现突出了神经电路布线特异性的动态细胞过程和分支行为.

科学领域:

神经科学
发育生物学
分子生物学

背景情况:

神经电路组装涉及到许多神经元过程的精确准.
这种精确的定位背后的动态细胞机制尚不清楚.

研究的目的:

调查Drosophila嗅觉电路中控制嗅觉受体神经元 (ORN) 轴突向的动态细胞过程.
了解ORN如何精确地向ipsi和逆侧天线叶中的球粒.

主要方法:

从30种ORN类型的单个轴突的时间间隔成像.
适应光学校正的格子光片显微镜.
上神经的切除.

主要成果:

在ORN轴突准中,延伸速度,内化时间和分支位置的多样性呈现出差异.
通过稳定短暂的间歇分支来实现ipsilateral目标.
目标地点的探索分支由基于微管的结构和富含F-actin的枢纽组成.
双边轴突和ORN轴突分别在反侧向和突触后伴侣精细化中发挥作用.

结论:

提供关于神经连接特异性的细胞洞察.
证明了精确的ORN轴突准的动态细胞机制.
突出了轴突相互作用在电路形成中的重要性.

关键词:

时间间隔成像, 电路组装, 嗅觉系统, 嗅觉受体神经元, 叶, , 细胞骨, 双边对称性, 目标选择

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Olfaction

Olfaction

The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...