生物分子凝聚物的核化景观
Shunsuke F Shimobayashi1, Pierre Ronceray2,3, David W Sanders1
1Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USA.
Nature
|September 23, 2021
概括
通过液相分离形成细胞凝结物. 这项研究表明核形成遵循与非生物系统相似的物理原理,
科学领域:
- 细胞生物学
- 生物物理
- 生物分子工程
背景情况:
- 细胞结构如核细胞通过生物分子的液相分离 (LLPS),特别是内在无序区域 (IDR) 形成.
- 经典核化理论 (CNT) 模型在非生物系统中的相分离,但其在复杂的细胞环境中的适用性尚不清楚.
研究的目的:
- 研究活细胞中的凝结核是否遵循与无生命系统相似的物理原理.
- 确定生物分子特征是否影响细胞凝结核的有效性和位置.
主要方法:
- 活细胞内源性和仿生凝聚物的核化动力学的定量表征.
- 应用类似于经典核化理论的框架来分析凝结物形成.
主要成果:
- 活细胞中的凝结核发生的物理过程类似于无生命物质.
- 生物分子特征,特别是IDR,可以作为相容的种子来增强核化速率.
- 细胞过程动力学影响凝结核化速率和空间特异性.
结论:
- 一个定量,类似于CNT的框架可以描述细胞内凝聚物核化.
- 核化部位的有效性可以根据生物分子特征进行调整.
- 这种理解使得合成凝聚物的精确空间和时间工程成为可能.
相关概念视频
Noncovalent Attractions in Biomolecules
18.8K
18.8K
Phase Transitions: Vaporization and Condensation
19.4K
The physical form of a substance changes on changing its temperature. For example, raising the temperature of a liquid causes the liquid to vaporize (convert into vapor). The process is called vaporization—a surface phenomenon. Vaporization occurs when the thermal motion of the molecules overcome the intermolecular forces, and the molecules (at the surface) escape into the gaseous state. When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase...
19.4K
Colloidal precipitates
1.1K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
1.1K
Recrystallization: Solid–Solution Equilibria
1.5K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.5K
Mechanisms of Membrane Domain Formation
3.4K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.4K
Energetics of Solution Formation
7.0K
The formation of a solution is an example of a spontaneous process, which is a process that occurs under specified conditions without energy from some external source.
When the strengths of the intermolecular forces of attraction between solute and solvent species in a solution are no different than those present in the separated components, the solution is formed with no accompanying energy change. Formation of the solution requires the solute–solute and solvent–solvent...
When the strengths of the intermolecular forces of attraction between solute and solvent species in a solution are no different than those present in the separated components, the solution is formed with no accompanying energy change. Formation of the solution requires the solute–solute and solvent–solvent...
7.0K


