传感器介导的ROS清理抑制了的免疫力,并被一种真菌效应器利用
Mingjun Gao1, Yang He1, Xin Yin2
1National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
Cell
|October 1, 2021
概括
植物使用一个以Ca2+传感器ROD1.1为中心的保存网络微调免疫平衡. 这一发现提供了通过整合Ca2+感应和ROS恒温的方法来培育抗病,高产的作物.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 植物病理学 植物病理学
背景情况:
- 构成性活跃的植物免疫可以危及生长和产量.
- 了解天然植物息地中的免疫恒温至关重要.
- 谷物具有保留的免疫抑制网络.
研究的目的:
- 为了研究谷物中免疫恒温的分子机制.
- 为了确定植物免疫反应的关键调节者.
- 探索提高作物抗病能力的策略,而不会影响产量.
主要方法:
- 对Ca2+传感器的表征 米对疾病的抗性1 (ROD1).
- 分析ROD1在活性氧物种 (ROS) 清除和催化酶活性中的作用.
- 研究ROD1无化和蛋白质稳定性的研究.
- 评估ROD1干扰和天然等位基对植物免疫力和产量的影响.
- 对ROD1.1的真菌效应剂AvrPiz-t模仿的结构分析.
主要成果:
- 确定ROD1是谷物中保存免疫抑制网络的核心组成部分.
- ROD1通过刺激催化酶活性来增强ROS清除,稳定性通过无处不在调节.
- 破坏ROD1会产生广泛的抗病能力.
- 印加大米中的天然ROD1等位基因增强了耐药性,而不会影响产量.
- 菌效因子AvrPiz-t模仿ROD1以抑制宿主免疫力并促进病毒性.
结论:
- 整合Ca2+感应和ROS稳态的分子框架抑制了宿主和病原体的植物免疫力.
- 该框架为开发抗病,高产作物提供了一个原则.
- 针对ROD1介导的途径提供了一种新的作物改进策略.
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