相关实验视频

Updated: Oct 17, 2025

Author Spotlight: Advancements in Multichannel Extracellular Recording for Studying Neuronal Activity in Freely Moving Mice

Author Spotlight: Advancements in Multichannel Extracellular Recording for Studying Neuronal Activity in Freely Moving Mice

Published on: May 26, 2023

小鼠主要运动皮层的转录和表观细胞图谱

Zizhen Yao¹, Hanqing Liu², Fangming Xie³

¹Allen Institute for Brain Science, Seattle, WA, USA.

|October 7, 2021

概括

研究人员创建了老鼠主要运动皮层的综合细胞图谱,确定了超过56种可再生的神经元细胞类型. 这种脑细胞图谱有助于理解脑细胞的组织,并设计有针对性的功能分析.

科学领域:

神经科学
基因组学
计算生物学

背景情况:

单细胞转录学提供了对大脑细胞多样性的分子见解.
整合多组数据用于细胞类型验证仍然是一个挑战.
小鼠的主要运动皮质对于运动至关重要,并且在进化过程中得到保护.

研究的目的:

创建一个高分辨率的小鼠原发动机皮层的分子和基因组图谱.
开发和应用计算方法来整合多式单细胞数据.
识别和验证可复制的细胞类型及其调节元素.

主要方法:

从50多万个小鼠脑细胞生成了转录组和表观组.
开发了多式联网数据集成的新计算和统计方法.
通过不同方法和技术量化验证细胞类型的可复制性.

主要成果:

建立了一个详细介绍超过56种不同且高度可复制的神经细胞类型的参考地图.
发现了类似于第四层金字塔细胞的刺激神经元群.
发现了成千上万的一致标记基因和基因调控元素,用于确定细胞类型.

结论:

小鼠的主要运动皮质表现出复杂的细胞类型分子调节.
生成的地图集为大脑细胞类型组织提供了全面的资源.
这项工作可以开发针对细胞类型的功能研究的试剂.

更多相关视频

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Published on: June 13, 2017

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

相关概念视频

Motor and Sensory Areas of the Cortex

Motor and Sensory Areas of the Cortex

The cerebral cortex, the brain's outermost layer, is pivotal in processing complex cognitive tasks, emotions, and various sensory inputs and executing voluntary motor activities. This intricate structure is divided into three primary functional areas: the motor areas, sensory areas, and association areas.
Motor Areas
The motor areas located in the frontal lobe are central to controlling voluntary movements. This region is further subdivided into the primary motor cortex and the premotor cortex....