一个神经解剖学的基础,用于电驱动 - 上腺轴
Shenbin Liu1,2,3,4, Zhifu Wang1, Yangshuai Su1,5
1Dana-Farber Cancer Institute and Department of Neurobiology, Harvard Medical School, Boston, MA, USA.
特定的感觉神经元 (PROKR2Cre) 对于电来说至关重要,以减少通过阴道 - 上腺轴的炎症. 切除这些神经元阻断ES的抗炎作用,揭示了针点特异性的神经解剖学基础.
科学领域:
- 神经科学
- 自主神经系统
- 免疫学
背景情况:
- 体感自主反射使得电刺激 (ES) 能够远程影响生理学,自20世纪70年代以来观察到身体区域的特异性.
- 例如,ES在后肢ST36针点,但不是腹部ST25针点,激活小鼠的上腺抗炎轴.
- 这种自主反射体组织的底层神经解剖学基础仍然不清楚.
研究的目的:
- 阐明体感自主反射的身体区域特异性的神经解剖学基础.
- 通过阴道 - 上腺轴识别负责介导电刺激的抗炎作用的特定感官神经元.
主要方法:
- 使用PROKR2Cre标记的感觉神经元,这些神经元激活深部后肢带膜,但不是腹部带膜.
- 研究了切除这些特定神经元对上腺轴激活和ES后全身炎症抑制的影响.
- 使用PROKR2Cre神经终端的光遗传刺激来评估它们在驱动自主路径中的作用.
主要成果:
- 具有PROKR2Cre标记的感觉神经元对于驱动阴道-上腺轴至关重要;它们的切除阻止了ES诱导的阴道效应神经元激活和catecholamine释放.
- 在缺乏这些特定感官神经元的小鼠中,ES未能抑制内毒素诱导的全身炎症.
- 高强度ES唤起的脊髓交感反射不受移除的影响,而PROKR2Cre终端的光遗传刺激选择性地激活了上腺轴.
结论:
- 具有PROKR2Cre标记的感觉神经元提供了针点刺激在激活上腺通路的特异性的神经解剖基础.
- 这一发现解释了如何在特定的身体区域,如ST36针点,低强度ES有效地减少全身炎症.
- PROKR2Cre神经纤维的分布可以预测ES在诱导抗炎作用方面的有效性.
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