Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

6.7K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.7K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

80.7K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
80.7K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.3K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.3K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

4.0K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
4.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structure and mechanism of the HSV-1 origin-binding protein UL9.

Journal of virology·2026
Same author

Ultra-rapid cryo-EM data acquisition method enabled by continuous recording based beam image shift.

Nature communications·2026
Same author

LRP4 is an entry receptor for multiple encephalitic alphaviruses.

Nature communications·2026
Same author

NIR-II Fluorescent Nanoplatforms with Defect-Regulated Piezoelectricity for Dual NIR-II/MRI-Guided, Hypoxia-Resilient Piezo-Chemodynamic Therapy and cGAS-STING Activation in Orthotopic Liver Tumor.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Severity-dependent risk of chromosomal abnormalities in fetuses with short long bones: a 10-year cohort study.

Human genomics·2026
Same author

Artificial intelligence for traumatic brain injury imaging: a translational review from algorithm development to clinical implementation.

Frontiers in neurology·2026

相关实验视频

Updated: Oct 17, 2025

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

9.7K

委内瑞拉马脑炎病毒及其受体LDLRAD3的结构

Bingting Ma1, Cuiqing Huang2,3, Jun Ma2

  • 1Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Center for Infectious Disease Research, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, P. R. China.

Nature
|October 14, 2021
PubMed
概括

委内瑞拉马脑炎病毒 (VEEV) 的进入是由LDLRAD3介导的. 结构分析显示了VEEV-LDLRAD3相互作用,为开发VEEV进入抑制剂提供了基础.

更多相关视频

In Vivo Imaging Systems IVIS Detection of a Neuro-Invasive Encephalitic Virus
10:21

In Vivo Imaging Systems IVIS Detection of a Neuro-Invasive Encephalitic Virus

Published on: December 2, 2012

21.0K
A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

6.7K

相关实验视频

Last Updated: Oct 17, 2025

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

9.7K
In Vivo Imaging Systems IVIS Detection of a Neuro-Invasive Encephalitic Virus
10:21

In Vivo Imaging Systems IVIS Detection of a Neuro-Invasive Encephalitic Virus

Published on: December 2, 2012

21.0K
A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

6.7K

科学领域:

  • 结构生物学
  • 病毒学
  • 分子生物学

背景情况:

  • 委内瑞拉马脑炎病毒 (VEEV) 在人类和马匹中引起严重的脑炎.
  • 目前没有针对VEEV感染的疫苗或治疗方法.
  • 低密度脂蛋白受体A类含域3 (LDLRAD3) 是一种新发现的VEEV宿主细胞受体.

研究的目的:

  • 确定与VEEV复合的LDLRAD3域1 (LDLRAD3-D1) 的冷电子显微镜结构.
  • 为了阐明LDLRAD3和VEEV之间的分子相互作用.
  • 为了确定VEEV进入抑制剂的潜在目标.

主要方法:

  • 低温电子显微镜 (cryo-EM) 用于确定高分辨率的结构.
  • 结构分析以确定绑定接口和相互作用.
  • 针对位点的突变发生,以调查关键的残留物和结合性亲缘关系.

主要成果:

  • 确定了LDLRAD3-D1与VEEV病毒样粒子结合的3.0 Å冷-EM结构.
  • 透露了LDLRAD3-D1的软木结构与VEEV E2-E1异构体通过疏水和极性接触相互作用.
  • 确定了对VEEV结合至关重要的特定LDLRAD3- D1残留物;一些突变物表现出增强的结合亲和力.

结论:

  • 该结构为VEEV-LDLRAD3相互作用提供了原子层面的洞察力.
  • LDLRAD3-D1 作为开发 VEEV 进入抑制剂的潜在支架.
  • 这些发现有助于更好地了解阿尔法病毒的组合和受体结合,指导治疗的发展.