警外表的存在!
Filippo Calzolari1, Benedikt Berninger2
1Institute of Physiological Chemistry, University Medical Center Mainz, 55128 Mainz, Germany.
Cell
|October 15, 2021
概括
针对质细胞的工程腺相关病毒 (AAV) 在神经元中表现出意想不到的活性. 这一发现挑战了目前依赖于细胞转化为神经元的脑修复策略.
科学领域:
- 神经科学
- 分子生物学
- 复原医学
背景情况:
- 腺相关病毒 (AAV) 是广泛使用的基因传递载体.
- AAV通常用于细胞特异性表达,例如向质细胞.
- 质细胞转化为神经元是大脑修复的一个有前途的策略.
研究的目的:
- 调查用于质细胞特异性向的AAV体内表达模式.
- 确定这些AAV是否在内源神经元中表现出异常表达.
- 评估这种非目标表达对质转化研究的影响.
主要方法:
- 使用基因工程设计的AAV载体,用于特定的细胞质促进体.
- 在体内给模型系统的AAV.
- 进行详细的组织学和分子分析以检测细胞质和神经元中的病毒表达.
主要成果:
- 在内源性神经元中观察到AAV的显著和广泛表达,与预期的质细胞特异性向相反.
- 证明使用质特异性促进剂设计的AAV可以在神经元群体中激活.
- 确定了可能导致这种意外神经元活动的机制.
结论:
- 目前的细胞特异性AAV载体可能无法可靠地限制细胞表达.
- 工程化AAV的观察到的神经活动可能会混细胞转化到神经元的研究.
- 研究人员应谨慎地对神经元群体的AAV表达特征进行验证,以进行大脑修复应用.
相关概念视频
Hindsight Biases
4.0K
Hindsight bias leads you to believe that the event you just experienced was predictable, even though it really wasn’t. In other words, you knew all along that things would turn out the way they did. Can you relate this to the phrase "Hindsight is 20/20" now?
4.0K
Understanding Deception
21
Deception is a pervasive aspect of human communication. Empirical studies have shown that most individuals engage in some form of deceit on a daily basis, with approximately 20% of social exchanges involving deceptive elements. Lying follows a developmental trajectory, peaking during adolescence and declining with age, possibly due to the maturation of cognitive control and social accountability.Cognitive and Social Factors in Deception DetectionDespite its prevalence, accurately detecting...
21
Perceptual Constancy
697
Perceptual constancy is the ability to recognize that objects remain consistent and unchanged even when their appearance varies due to changes in sensory input. There are four main types of perceptual constancy: size constancy, shape constancy, color constancy, and brightness constancy.
Size constancy is the recognition that an object remains the same size, even when its image on the retina changes. For instance, a bus is perceived to be large enough to carry people, even if it looks tiny from...
Size constancy is the recognition that an object remains the same size, even when its image on the retina changes. For instance, a bus is perceived to be large enough to carry people, even if it looks tiny from...
697
Nonconscious Mimicry
4.7K
Nonconscious mimicry occurs when individuals alter their mannerisms to match the behaviors and expressions of those nearby, without intention.
4.7K
The Availability Heuristic
6.6K
A heuristic is a general problem-solving framework (Tversky & Kahneman, 1974). You can think of these as mental shortcuts that are used to solve problems. Different types of heuristics are used in different types of situations, and the impulse to use a heuristic occurs when one of five conditions is met (Pratkanis, 1989):
6.6K
Impression Management Techniques I: Managing Appearances
33
Appearance is a multidimensional aspect of self-presentation that encompasses observable attributes such as clothing, grooming, speech, and nonverbal behavior. These elements are often strategically managed to align with socially constructed expectations in different settings. For instance, individuals tailor their appearance during job interviews, social gatherings, or athletic events to meet the perceived norms of those environments.Contextual Adaptation and Social SignalsThe research...
33


