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Olav Schiemann1, Caspar A Heubach1, Dinar Abdullin1

  • 1Institute of Physical and Theoretical Chemistry, University of Bonn, Wegelerstraße 12, 53115 Bonn, Germany.

Journal of the American Chemical Society
|October 19, 2021
PubMed
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这项研究建立了脉冲二极光谱的质量标准,提高了结构生物学模型的可靠性. 这些指导方针确保了蛋白质构造分析的精确距离分布数据.

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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