Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

15.3K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
15.3K
Telomeres and Telomerase02:41

Telomeres and Telomerase

24.9K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
24.9K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

2.6K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.6K
The Electron Transport Chain01:30

The Electron Transport Chain

18.1K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
18.1K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

3.9K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.9K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

8.3K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
8.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Inhibitory modulation of age-dependent behavior through <i>dsx</i>-expressing cells in honeybees.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Climate-driven in-situ trait variation in an annual ruderal grass across Europe.

Annals of botany·2026
Same author

Reply to letter regarding the article "Loss of cardiomyocyte AKT signaling causes deterioration of lipid metabolism and cellular atrophy".

Metabolism: clinical and experimental·2026
Same author

Loss of cardiomyocyte AKT signaling causes deterioration of lipid metabolism and cellular atrophy.

Metabolism: clinical and experimental·2026
Same author

Artificial soil (ArtSoil): Recreating soil conditions in synthetic plant growth media.

The Plant journal : for cell and molecular biology·2026
Same author

Combined high-fat, high-sucrose diet and streptozotocin treatment induces cardiometabolic heart failure with preserved ejection fraction in mice.

American journal of physiology. Heart and circulatory physiology·2026

相关实验视频

Updated: Oct 16, 2025

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
07:23

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

6.4K

线粒体端粒酶逆转录酶通过改善I复合体的组成和功能来保护心肌缺血/再生损伤

Niloofar Ale-Agha1, Philipp Jakobs1, Christine Goy1,2

  • 1Environmentally-induced Cardiovascular Degeneration, Clinical Chemistry and Laboratory Diagnostics (N.A.-A., P,J., C.G., M.Z., J.R., N.D.-R., J.G., F.v.A., O.E., J.A., J.H.), University Hospital and Heinrich-Heine University, Düsseldorf, Germany.

Circulation
|October 21, 2021
PubMed
概括

线粒体端粒酶逆转录酶 (TERT) 通过改善线粒体功能和减少心脏病发作大小来保护心脏免受损伤. 增加线粒体TERT可能为心脏保护提供新的治疗方法.

关键词:
转基因的小鼠线粒体心肌缺血症肌纤维细胞转血损伤长线酶

更多相关视频

Model of Ischemia and Reperfusion Injury in Rabbits
06:11

Model of Ischemia and Reperfusion Injury in Rabbits

Published on: November 3, 2023

1.4K
Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
07:40

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

1.3K

相关实验视频

Last Updated: Oct 16, 2025

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
07:23

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

6.4K
Model of Ischemia and Reperfusion Injury in Rabbits
06:11

Model of Ischemia and Reperfusion Injury in Rabbits

Published on: November 3, 2023

1.4K
Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
07:40

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

1.3K

科学领域:

  • 心血管生物学
  • 线粒体医学
  • 端粒生物学

背景情况:

  • 已知特洛马酶逆转录酶 (TERT) 具有心血管保护功能.
  • TERT定位在核和线粒体中,但其在每个隔间的具体作用尚不清楚.
  • 目前的研究缺乏区分核与线粒体TERT功能的工具.

研究的目的:

  • 阐明核和线粒体TERT在心血管保护中的不同作用.
  • 研究线粒体TERT对心脏功能和损伤反应的影响.
  • 探索针对线粒体TERT的治疗潜力.

主要方法:

  • 产生具有独家线粒体 (mitoTERT) 或核 (nucTERT) TERT表达的新型小鼠模型.
  • 在缺血/再输血损伤后的评估结果.
  • 评估了线粒体呼吸,心肌细胞亡,肌纤维细胞分化和内皮细胞功能.
  • 分析了线粒体复合I子单元的组成.

主要成果:

  • 线粒体TERT (线粒体TERT) 增强了心脏线粒体呼吸,降低了心脏病发作大小和射出分数在缺血/再输血后的下降.
  • 核TERT (nucTERT) 没有提供保护,而TERT缺乏导致线粒体呼吸功能受损.
  • 改善了心肌细胞存活率,肌纤维细胞分化和内皮细胞迁移.
  • 在人类心脏线粒体中发现了TERT,并且通过缺血预调增加.

结论:

  • 线粒体TERT,而不是核TERT,对于线粒体呼吸和防止缺血/再输伤害至关重要.
  • 线粒体TERT增强复合I功能并提供心脏保护.
  • 提升线粒体TERT水平为心血管疾病提供了一个有前途的治疗策略.