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Updated: Oct 16, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

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绘制DNA双链断裂修复的遗传景观

Jeffrey A Hussmann1, Jia Ling2, Purnima Ravisankar2

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

Cell
|October 21, 2021
PubMed
概括

我们使用Repair-seq绘制了DNA双链断裂 (DSB) 修复路径,揭示了基因编辑中的新基因关系和依赖性. 这促进了对基因组完整性的理解,并优化了基因编辑工具.

关键词:
在CRISPR-Cas9中DNA 修复双链断裂功能性基因组学基因组编辑

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