可光激活的光染料和它们在多色纳米学中的使用
Alexey N Butkevich1, Michael Weber2, Angel R Cereceda Delgado1,2
1Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.
Journal of the American Chemical Society
|October 29, 2021
概括
新的光染料具有性碳酸盐组,可在紫外线下快速光激活. 这些染料具有很高的水溶性,并且与超高分辨率显微镜技术兼容,用于精确的细胞成像.
科学领域:
- 化学生物学
- 显微镜
- 生物光学
背景情况:
- 开发新的光探测器对于超高分辨率显微镜的发展至关重要.
- 现有的探针可能受到水溶性限制或不必要的光激活的影响.
- 需要具有高特异性和与活细胞成像相容的可光激活染料.
研究的目的:
- 推出一种新的光染料系列,
- 为了证明它们在紫外线下的快速光激活和对虚假的双光子激活的抵抗力.
- 在多色超分辨率成像和精确的单分子定位中验证它们的实用性.
主要方法:
- 新型光染料的合成,其中含有水友性碳酸盐化基.
- 使用紫外线 (≤400 nm) 和高强度可见/红外线的光激活实验.
- 评估水溶性和转化为超分辨率和活细胞兼容的光体.
- 在多色刺激排放耗尽 (STED) 纳米镜中应用.
- 使用最小光谱电D (MINSTED) 和MINFLUX技术进行单一光体定位.
主要成果:
- 建议的染料在紫外线照射下表现出快速的光激活.
- 在高强度可见光或红外光下没有观察到虚假的两光子激活.
- 中的染料具有很高的水溶性,并转化为经过验证的超分辨率光体.
- 使用STED纳米镜成功获取多达六种颜色的图像.
- 使用MINSTED和MINFLUX实现了单个光体的精确定位,标准偏差为3nm.
结论:
- 开发的光染料为先进的光学显微镜提供了强大的平台.
- 它们的特定光激活特性和高水溶性增强了活细胞成像能力.
- 这些染料适用于多色超分辨率成像和高精度单分子定位技术.
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