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Updated: Oct 14, 2025

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完整活性的哺乳动物细胞的细胞内增强敏感性NMR

Rupam Ghosh1, Yiling Xiao1, Jaka Kragelj1

  • 1Department of Biophysics, UT Southwestern Medical Center, Dallas, Texas 75390-8816, United States.

Journal of the American Chemical Society
|November 1, 2021
PubMed
概括

动态核极化 (DNP) 增强了核磁共振 (NMR) 以检测活细胞中的蛋白质. 这项研究建立了DNP辅助MAS NMR的条件,使生物分子在完整的活细胞内进行结构研究.

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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