对外皮层投射神经元命运多样性的时间控制
Esther Klingler1, Ugo Tomasello1, Julien Prados2
1Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
Nature
|November 10, 2021
概括
新皮质投射神经元在出生后发展出不同的分子和功能特征. SOX11表达时间影响小鼠的感觉运动连接和探索行为,揭示了发育速度的差异.
科学领域:
- 神经科学
- 发育生物学
- 分子生物学
背景情况:
- 新皮层相互连接对于感官整合和感官运动转换至关重要.
- 区域间皮质投射神经元 (ICPN) 调解这些功能,但它们的发育起源尚不清楚.
- 现有研究显示ICPN的解剖学多样性但分子同质性.
研究的目的:
- 研究单个ICPN中解剖学和功能多样性的发育过程.
- 在小鼠新皮层中连接ICPN的连接和转录.
- 确定驱动ICPN亚型差异化的分子机制.
主要方法:
- 通过测序 (MAPseq) 和单细胞RNA测序进行多重分析.
- 从发育中的小鼠的主要体感皮质 (S1) 中分析单个ICPN.
- 通过产后操纵其表达来研究SOX11的作用.
主要成果:
- 对运动皮层投射与二次体感皮层投射的ICPN显现出不同的产后分子和功能差异化步伐.
- 确定了SOX11作为这些ICPN亚型之间差异表达的关键转录因子.
- 证明S1中的SOX11操纵会影响传感运动连接和探索行为.
结论:
- 不同的ICPN亚型表现出不同的产后分子分化率,影响电路连接性和功能.
- 时间基因表达动态,特别是SOX11,有助于皮层神经元的类型内多样性.
- 发展速度,而不是不同的遗传程序,可能是ICPN多样性的基础.
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