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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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通过V型CRISPR转位子系统选择和重塑目标部位

Irma Querques1, Michael Schmitz1, Seraina Oberli1

  • 1Department of Biochemistry, University of Zurich, Zurich, Switzerland.

Nature
|November 11, 2021
PubMed
概括

研究人员揭示了由CRISPR相关的转位子引导DNA转位的机制. 结构和生化研究表明Cas12k如何引导DNA插入,使新的基因编辑工具成为可能.

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