Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.8K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.8K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.0K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.0K
Heterochromatin02:38

Heterochromatin

15.0K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
15.0K
Euchromatin01:01

Euchromatin

7.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
7.9K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

6.2K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
6.2K
Histone Modification02:32

Histone Modification

14.7K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
14.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Oncogene inactivation-induced senescence facilitates tumor relapse.

Nature communications·2026
Same author

Haplotype-resolved genome architecture mapping uncovers pervasive structural heterogeneity between human homologous chromosomes.

bioRxiv : the preprint server for biology·2026
Same author

Emergent Isotropic-Nematic Transition in 3D Semiflexible Active Polymers.

Physical review letters·2026
Same author

Emergent domain segregation in self-interacting polymers explains chromosome 3D conformations in single human cells.

Physical review. E·2026
Same author

Physics-Based Modeling of Sparse Single-Cell Hi-C Uncovers Structural and Epigenetic Variability.

International journal of molecular sciences·2026
Same author

Supporting-like cells constitute an alternative steroidogenic lineage conserved in amniotes.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Oct 13, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.8K

细胞类型的专业化由特定的染色体拓编码

Warren Winick-Ng1, Alexander Kukalev2, Izabela Harabula2,3

  • 1Max-Delbrück Centre for Molecular Medicine, Berlin Institute for Medical Systems Biology, Epigenetic Regulation and Chromatin Architecture Group, Berlin, Germany. warren.winick-ng@mdc-berlin.de.

Nature
|November 18, 2021
PubMed
概括

我们开发了immunoGAM来绘制特定类型脑细胞的3D染色体结构, 这种方法揭示了染色体组织如何与基因表达和特殊的神经元功能有关.

更多相关视频

TChIP-Seq: Cell-Type-Specific Epigenome Profiling
07:28

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

8.0K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.5K

相关实验视频

Last Updated: Oct 13, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.8K
TChIP-Seq: Cell-Type-Specific Epigenome Profiling
07:28

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

8.0K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.5K

科学领域:

  • 基因组学和分子生物学
  • 神经科学
  • 表观遗传学

背景情况:

  • 三维 (3D) 染色体结构对于基因调节和细胞功能至关重要.
  • 现有的染色体构成捕获方法在绘制神经系统方面存在局限性,特别是在没有组织解离的情况下对动态变化和细胞类型的特异性.

研究的目的:

  • 开发和应用一种新的方法,即 immunoGAM,用于在单个动物的特定脑细胞类型中绘制全基因组的3D染色素拓.
  • 研究细胞类型的3D染色体结构与大脑中的基因表达模式之间的关系.

主要方法:

  • 免疫GAM是基因组架构映射 (GAM) 的扩展,利用核冷切割的无绑定技术来映射基因组拓.
  • 它可以从复杂的组织中选择细胞类型,使用较低的细胞数量 (约. 避免组织分离.
  • 通过分析跨核切片的DNA位点的共同分离概率来确定染色体相互作用.

主要成果:

  • 细胞类型的专用3D染色体结构在多个基因组尺度上绘制,与基因表达模式相关联.
  • 在高表达和/或高染色体可访问性期间观察到长基因的广泛"融化".
  • 神经元亚型特定的接触涉及与成和突触可塑性相关的基因,在可访问的染色体中具有转录因子结合位.
  • 感官受体基因被发现在异色区间中,形成长距离接触.

结论:

  • 在单个动物分辨率下研究特定脑细胞的3D染色体组织提供了一个强大的工具.
  • 大脑细胞中的特定染色体结构与基因调节机制和专门的细胞功能密切相关.
  • 这些发现突显了3D基因组架构在神经元细胞身份和功能中的重要性.