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针对非修改的mRNA COVID-19疫苗的非编码区域的优化

Makda S Gebre¹, Susanne Rauch², Nicole Roth³

¹Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

|November 18, 2021

概括

与非人类灵长类的第一代CVnCoV疫苗相比,第二代CureVac mRNA疫苗 (CV2CoV) 的免疫性和防护性得到改善.

科学领域:

疫苗学
免疫学
病毒学

背景情况:

第一代CureVac mRNA疫苗 (CVnCoV) 针对严重急性呼吸系统综合征冠状病毒-2 (SARS-CoV-2) 在人体试验中进行了评估.
CV2CoV是一种优化的第二代mRNA疫苗,具有增强的抗原表达和修改的非编码区域.

研究的目的:

为了比较CVnCoV和CV2CoV的免疫性和保护功效.
评估CV2CoV对SARS-CoV-2变种的性能,并将其与BNT162b2疫苗进行比较.

主要方法:

用两剂CVnCoV,CV2CoV或假的疫苗接种了Cynomolgus.
通过测量抗体标位,B细胞反应和T细胞反应来评估免疫性.
在SARS-CoV-2挑战后评估了保护效果,测量了呼吸道中的病毒载量.

主要成果:

与CVnCoV相比,CV2CoV诱导的结合和中和抗体标位,记忆B细胞和T细胞反应显著提高.
CV2CoV对包括德尔塔在内的SARS-CoV-2变种表现出强大的中和抗体反应.
CV2CoV对SARS-CoV-2的挑战提供了更强大的保护,病毒载量较低,并且与的BNT162b2具有相似的免疫性.

结论:

在mRNA疫苗中优化非编码区域可以显著提高免疫性和保护性.
与CVnCoV相比,CV2CoV是一个更有效的mRNA疫苗候选者.
抗体标位与对抗SARS-CoV-2感染的保护功效相关.

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