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厚组织的EASI-FISH定义了下丘脑的横向空间分子组织

Yuhan Wang¹, Mark Eddison¹, Greg Fleishman¹

¹Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

|December 7, 2021

概括

扩展辅助代在位混合 (EASI-FISH) 绘制大脑厚度部分的细胞类型. 这种方法揭示了横向下丘脑区域的九个不同的子区域, 推动了大脑组织研究.

科学领域:

神经科学
分子生物学
计算生物学

背景情况:

描述大脑结构需要了解分子定义的细胞类型的空间组织和形态.
目前的方法在测量大组织体积的基因表达时遇到瓶.
侧下垂体区域 (LHA) 的解剖组织定义不佳.

研究的目的:

开发一种用于测量大脑厚处基因表达的新方法.
创建一个计算管道来处理由新方法生成的大图像数据集.
研究LHA内的细胞类型的空间分布,并确定其子区域.

主要方法:

开发了扩展辅助代在位混合 (EASI-FISH) 用于在300μm大脑部分进行基因表达分析.
为快速处理EASI-FISH图像数据创建了一个计算管道.
应用了EASI-FISH来绘制几十种分子定义的细胞类型在侧面的下丘脑区域.

主要成果:

EASI-FISH能够重建跨大脑的空间分子域.
在LHA中绘制细胞类型揭示了九个空间和分子定义的子区域.
对scRNA-seq数据集的代再分析确定了解决空间和形态异质性的标记基因.

结论:

EASI-FISH提供了一个可扩展的方法来绘制大脑组织中分子定义的细胞类型.
开发的计算管道使细胞类型的映射变得民主化,并促进大脑组织的发现.
这种技术提高了对LHA等复杂大脑区域的理解.

关键词:

三维细分在现场的光混合一个RNA 大脑大脑解剖学细胞类型扩展显微镜侧向下丘脑转录组学

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