相关实验视频
Updated: Aug 17, 2026

11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
概括
研究人员在小鼠T细胞受体 (TCR) β链转录中发现了一种新的替代RNA拼接途径,揭示了可选的72个基对的外因子插入. 这一发现扩大了我们对免疫细胞中基因表达调节的理解.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
背景情况:
- 替代RNA处理从单个基因中产生多种不同的信使RNA物种.
- 免疫球蛋白和T细胞受体 (TCR) 基因表达涉及复杂的拼接事件.
- 之前的研究记录了免疫球蛋白重链和可变区域表达的替代拼接.
研究的目的:
- 研究在小鼠T细胞受体 (TCR) β链转录中的替代RNA拼接.
- 识别和描述TCRβ链基因表达中的新型拼接事件.
主要方法:
- 从TCRβ链转录获得的互补DNA (cDNA) 克隆的序列分析.
- 在TCRβ链中插入的序列的识别. 补充DNA.
主要成果:
- 确定了一种新型的Cβ1信使RNA物种,该物种在连接 (Jβ) 和常数 (Cβ) 元素之间含有72个基对 (bp) 插入.
- 这种插入来自于特定于Cβ1转录的替代拼接途径.
- 在没有Cβ1基因的小鼠中,可选的外基因可以与Cβ2结合.
- 替代拼接途径的使用率从1%到18%不等.
结论:
- 对于小鼠T细胞受体 (TCR) β链转录,存在一种以前未知的替代RNA拼接途径.
- 这一途径将72bp的可选外子引入到Cβ1转录中,增加了TCRβ链表达的复杂性.
- 这一发现为控制TCR基因表达和免疫细胞发育的调节机制提供了新的见解.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Editing
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
RACE - Rapid Amplification of cDNA Ends
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
Since the...

