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相关概念视频

Proteomics01:33

Proteomics

8.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.4K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

7.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
7.2K

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相关实验视频

Updated: Oct 9, 2025

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

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向单分子蛋白质组学发展

Filip Bošković1, Ulrich F Keyser1

  • 1Cavendish Laboratory, University of Cambridge, Cambridge, UK.

Science (New York, N.Y.)
|December 16, 2021
PubMed
概括
此摘要是机器生成的。

纳米孔测序现在可以分析单个蛋白质,为先进的蛋白质组学铺平道路. 这一突破开启了蛋白质分析和发现的新时代.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

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相关实验视频

Last Updated: Oct 9, 2025

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.3K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

4.1K

科学领域:

  • 生物化学
  • 分子生物学
  • 分析化学

背景情况:

  • 传统的蛋白质组学方法在吞吐量和灵敏度方面存在局限性.
  • 在单个分子水平上分析完整的蛋白质是一个重大挑战.

研究的目的:

  • 引入和验证纳米孔重读作为单蛋白分析的新技术.
  • 展示纳米孔技术在促进蛋白质组学的潜力.

主要方法:

  • 使用纳米孔传感来检测和描述单个蛋白质分子.
  • 在纳米孔内开发蛋白质转移和信号解释的方法.

主要成果:

  • 证明了单个蛋白质的成功纳米孔重读.
  • 展示了区分不同蛋白质及其修饰的能力.

结论:

  • 纳米孔重读代表了下一代蛋白质组学的变革性方法.
  • 这种技术为蛋白质的识别和表征提供了前所未有的机会.