相关实验视频
Updated: May 14, 2026

13:10
Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
Published on: September 9, 2010
概括
这项研究引入了一种新的V-(D) -J重组测定方法,表明它发生在染色体上下文独立,可能对DNA排序不敏感.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 免疫球蛋白可变域通过V-(D) -J重组组合组合在一起.
- 这个过程涉及特定的DNA段和重组信号.
研究的目的:
- 开发一种灵敏,快速的测定V-D-J重组的方法.
- 为了研究V-(D) -J重组的染色体背景独立性.
主要方法:
- 一种新的测定方法,使用在B细胞前引入的等离子体DNA.
- 对包括删除和反转在内的重组事件的分析.
主要成果:
- 在V-(D) -J重组试验是敏感和快速.
- 重组是独立于独特的染色体背景.
- 特定的侧翼序列包含必要的重组信号.
- 删除和反转发生在类似的频率.
结论:
- V-(D) -J重组是强大的,不依赖于特定的染色体位置.
- 复合点的拓布局可能不会显著影响该过程.
- 新形成的交叉点上的事件可能相互连接.
相关概念视频
Nucleotide Excision Repair
Overview
Base Excision Repair
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
The first step of...
Long-patch Base Excision Repair
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Base Excision Repair
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
The first step of...
Nucleotide Excision Repair
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...

