对活细胞进行生物对等蛋白质标记的超快四-BCN循环添加反应
Gangam Srikanth Kumar1, Stefano Racioppi1, Eva Zurek1
1Department of Chemistry, State University of New York at Buffalo, Buffalo, New York 14260-3000, United States.
Journal of the American Chemical Society
|December 29, 2021
概括
研究人员使用硫化四醇和双环[6.1.0]non-4-yn-9-ylmethanol (BCN) 开发了一种快速生物对接反应剂对. 这种新方法可以在几秒钟内对活细胞进行超快速的生物对角标记.
科学领域:
- 生物对角化学
- 化学生物学
- 有机合成
背景情况:
- 对于研究生物系统而言,生物直角反应至关重要.
- 现有的方法往往缺乏实时蜂标签所需的速度和效率.
- 基于醇的循环添加物是有前途的,但可能受到竞争反应的限制.
研究的目的:
- 设计和合成一种新型的超快速生物对立反应剂对.
- 使用计算方法研究新反应物对的动力优势.
- 为了证明新一对对细胞标记的有用性.
主要方法:
- 用N-硫烯基替代剂设计和合成化屏蔽的硫化四.
- 使用bicyclo[6.1.0]non-4-yn-9-yl甲醇 (BCN) 作为反应伙伴.
- 使用第一原理计算来分析反应动力学.
- 生细胞成像实验用于生物对角标记.
主要成果:
- 开发了一种具有优异反应动力学的新型四-BCN反应物对.
- 计算研究揭示了增强的循环添加率的起源.
- 在15秒内,四醇-BCN结合使得活细胞上的葡萄糖受体能够快速标记.
结论:
- 开发的四醇-BCN系统代表了生物对等化学的重大进步.
- 这种超快速结合使得细胞标签的时间分辨率前所未有.
- 这些发现为实时生物过程监测开辟了新的途径.
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