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菌根基因表达变化的影响在Fabricius的股票中的B细胞发育

C B Thompson¹, E H Humphries, L M Carlson

¹Fred Hutchinson Cancer Research Center, Seattle, Washington.

|November 6, 1987

概括

鸟类B细胞的发育涉及菌株基因放松调节,导致bursal干细胞的增殖. 这些细胞表现出胚胎毛囊淋巴细胞的特征,并有效地重新填充毛囊.

科学领域:

免疫学免疫学免疫学
发育生物学发展生物学
病毒学病毒学

背景情况:

布里西斯的毛囊对于鸟类B细胞发育至关重要.
Myc基因表达对细胞增殖和分化至关重要.
了解B细胞发育中的干细胞动态是必不可少的.

研究的目的:

为了研究复原病毒v-myc表达对鸟类bursal淋巴细胞的影响.
为了确定是否放松了菌根表达的影响bursal干细胞特征.
为了分析表达v-myc的bursal细胞的重新繁殖能力.

主要方法:

感染了18天的胚胎毛囊淋巴细胞与v-myc复原病毒.
表面免疫球蛋白阳性 (slg+) 细胞增殖的分析.
在经过化学切除的 bursae 中评估 bursal 细胞的重新填充.
免疫球蛋白轻链基因多样化的评估.

主要成果:

V-myc表达诱导了 bursa 中 slg+ 细胞的多克隆增殖.
与正常细胞相比,表达V-myc的bursal细胞显示出增强的再生效率.
在化四周后,正常的毛细胞失去了重新繁殖的能力.
抗菌基因 (v-myc或c-myc) 的放松调节保留了免疫球蛋白轻链多样化.

结论:

在鸟类B细胞发育中的真菌表达的逆转录病毒放松调节促进了bursal干细胞的外生长.
这些诱导细胞保留了bursal干细胞的主要表型特征.
这表明,通过真菌放松调节产生干细胞种群的机制.

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