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在SARS-CoV-2感染过程中唾液RT-PCR敏感性

Anne L Wyllie1, Prem K Premsrirut2

  • 1Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut.

JAMA
|January 11, 2022
PubMed
概括

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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