以自然历史为指导的奥米克揭示了植物对叶虫害的防御化学反应
Yuechen Bai1, Caiqiong Yang1, Rayko Halitschke1
1Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, D-07745 Jena, Germany.
概括
研究人员发现了一种新的植物防御机制, 这涉及到一种特殊的化合物, 保护作物, 提供一种新的害虫控制策略.
科学领域:
- 植物生物学
- 化学生态学
- 农业昆虫学
背景情况:
- 植物的非宿主耐药性对病原体有很好的研究,但对食草动物的了解较少.
- 恩波斯卡叶是一种重要的农业害虫,利用植物莉酸 (JA) 介导的信号来定位宿主.
研究的目的:
- 调查本地烟草中对Empoasca叶的非宿主耐药性的机制.
- 识别新的植物防御化合物和对草食动物产生抵抗力的途径.
主要方法:
- 使用基因组,转录组和代谢组分析对原生烟草的前向和逆向遗传线进行查.
- 一种新生物化学途径的体外复制,其中涉及多氧化酶和柏柏林桥梁酶2 (BBL2).
- 在作物中引入已识别的抗药机制.
主要成果:
- 在烟草中的Empoasca诱导的斯酸模块的识别.
- 发现和复制一种新的防御化合物:咖啡绿叶挥发物.
- 证明这种与BBL2合成的化合物能够在人工农作物中抵抗虫.
结论:
- 在本地烟草中已经阐明了对食草动物的保护性非宿主抵抗机制.
- 发现的JA-JAZi模块及其相关的挥发性化合物是作物保护战略的有希望的目标.
- 自然历史引导的前进遗传学为发现植物防御机制提供了有价值的方法.
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