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相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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相关实验视频

Updated: May 8, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

在c-abl mRNA中的替代5'外因子.

Y Ben-Neriah, A Bernards, M Paskind

    Cell
    |February 28, 1986
    PubMed
    概括

    细胞ABL原瘤基因,一种蛋白质-氨酸激酶,由于替代拼接,显示出不同的mRNA大小. 这种拼接发生在与费城染色体转位有关的同一个部位.

    科学领域:

    • 分子生物学分子生物学
    • 瘤发生的发生因子.
    • 基因表达 基因表达

    背景情况:

    • 细胞的abl原瘤基因编码了一个蛋白质-氨酸激酶.
    • 该基因在各种细胞类型中表达,通常是两个或三个不同的mRNA大小变异.

    研究的目的:

    • 为了研究小鼠c-abl cDNAs的结构多样性.
    • 了解产生c-ablmRNA异质性的机制.
    • 探索c-abl拼接和瘤转化之间的关系.

    主要方法:

    • 从70Z/3淋巴细胞中克隆了四种类型的小鼠c-abl互补DNA (cDNA).
    • 对克隆cDNA的5'序列进行分析,以确定预测的N端氨基酸序列.
    • 将c-abl cDNA序列与病毒abl (v-abl) 序列进行比较.

    主要成果:

    • 确定了四种不同的小鼠c-abl cDNA,每个都有独特的5'序列,编码了20-45个氨基酸的预测N-终端区域.
    • 一个cDNA与v-abl的gag N终端共享了一个met-gly-gln N终端序列.
    • 在c-ablcDNA中观察到的5'异质性归因于替代性添加5'外因子到3'外因子的共同集合中.
    • 发现,在费城染色体转位中,替代拼接发生在相同的位置,BCR序列附着在ABL序列上.

    更多相关视频

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
    09:58

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

    Published on: December 9, 2016

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

    相关实验视频

    Last Updated: May 8, 2026

    Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
    11:02

    Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

    Published on: May 27, 2016

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
    09:58

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

    Published on: December 9, 2016

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

    结论:

    • 细胞ABL原瘤基因在mRNA水平上表现出显著的结构多样性.
    • 替代拼接是产生不同形式的c-abl.的关键机制.
    • 涉及c-abl异质性的剪接部位在癌症转位的背景下被保存,例如费城染色体.