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相关概念视频

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.4K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.4K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.6K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.6K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.0K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.0K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.8K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.8K

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相关实验视频

Updated: Oct 4, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
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The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

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空间-CUT&Tag:在细胞水平上进行空间分辨的染色体修饰分析

Yanxiang Deng1,2, Marek Bartosovic3, Petra Kukanja3

  • 1Department of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.

Science (New York, N.Y.)
|February 10, 2022
PubMed
概括

随着空间CUT&Tag的发展,可以在组织中绘制全基因组基因组修饰图. 这项技术揭示了小鼠胚胎和大脑的表观遗传调节,为细胞的发展和命运提供了洞察力.

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Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
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Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

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Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
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Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

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相关实验视频

Last Updated: Oct 4, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
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The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

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Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
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Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
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科学领域:

  • 基因组学
  • 表观遗传学
  • 空间生物学

背景情况:

  • 空间奥米学是生物和生物医学研究中的一个快速发展的领域.
  • 了解表观遗传修饰的空间组织对于破译细胞功能和发育至关重要.

研究的目的:

  • 引入空间CUT&Tag,这是一个用于空间解析全基因组基因组修饰的新方法.
  • 在小鼠胚胎和大脑中展示空间CUT&Tag的应用,以调查表观遗传调节和细胞模式.

主要方法:

  • 在现场CUT&Tag化学与微流体确定性条形码和下一代测序的整合.
  • 在组织样本中应用空间CUT&Tag进行全基因组基因组修饰分析.
  • 使用免疫光成像识别20微米像素内的单个核,用于单细胞表观基因组导出.

主要成果:

  • 在小鼠胚胎中绘制出空间分辨的染色质状态,揭示了特定组织的表观遗传规则.
  • 在组织尺度上获得空间信息,与ENCODE引用相关.
  • 在小鼠大脑中阐明了皮层发育和空间细胞类型模式的表观遗传控制.
  • 从空间分析数据中成功地获得单细胞表观基因组.

结论:

  • 空间CUT&Tag为组织中的高分辨率空间表观基因组分析提供了强大的工具.
  • 这种方法为研究表观遗传调节,细胞功能和在正常生理和疾病中的命运决定提供了新的机会.
  • 这种技术通过在原生组织环境中对染色质状态进行详细分析,从而推进空间奥米克领域.