在缺乏光系统II的蓝藻中以电化学驱动的光合作用电子传输
Christine M Lewis1,2,3, Justin D Flory2,4, Thomas A Moore1,5
1School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287, United States.
Journal of the American Chemical Society
|February 14, 2022
概括
一个新的微生物电光合成系统 (MEPS) 绕过受损的光系统II (PSII) 驱动光合作用电极. 这种系统增强了电子传输以产生能量,从而提高了光合作用效率.
科学领域:
- 生物化学
- 光合作用研究
- 生物能源
背景情况:
- 光系统II (PSII) 对于光合作用中的水分解至关重要,但容易受到光损伤.
- 开发替代方法来驱动光合作用电子运输对于理解和改进光合作用至关重要.
研究的目的:
- 研究一种新的微生物电光合成系统 (MEPS),用于驱动缺乏功能PSII的Synechocystis细胞中的电子传输.
- 评估MEPS中依赖光的电子传递的效率和特性.
主要方法:
- 使用微生物电光合成系统 (MEPS) 带有氧化还原介质和电极来驱动*Synechocystis* (Δ*psbB*) 细胞中的电子运输.
- 测量了依赖光的电流产生和P700+的再减少动力学.
- 量化电子输送速率和电流密度.
主要成果:
- MEPS产生了一种依赖光的电流,随着光强度的增加.
- 电子传递率达到113微米电子h-1 mg-chl-1 在2050微米光子m-2s-1.
- 最初的电子递送率超过了野类PSII所驱动的,在细胞染色体*b*6*f*复合体之前发生,以支持NADPH和ATP的产生.
结论:
- MEPS成功地驱动了PSII缺乏的细胞中的光合作用电子运输.
- 这个系统作为基础光合作用研究的平台.
- MEPS显示在高光条件下增强光合作用性能.
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