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相关概念视频

Peroxisomes01:24

Peroxisomes

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Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
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Protein Import into the Peroxisomes01:27

Protein Import into the Peroxisomes

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Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
3.8K
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.2K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
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Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

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Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
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Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids02:04

Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids

6.2K
Diols are compounds with two hydroxyl groups. In addition to syn dihydroxylation, diols can also be synthesized through the process of anti dihydroxylation. The process involves treating an alkene with a peroxycarboxylic acid to form an epoxide. Epoxides are highly strained three-membered rings with oxygen and two carbons occupying the corners of an equilateral triangle. This step is followed by ring-opening of the epoxide in the presence of an aqueous acid to give a trans diol.
6.2K
Oxidation and Reduction of Organic Molecules01:19

Oxidation and Reduction of Organic Molecules

8.0K
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
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相关实验视频

Updated: Oct 3, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

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直接从序列设计的稳定且功能多样化的多功能过氧化物

Shiran Barber-Zucker1, Vladimir Mindel1, Eva Garcia-Ruiz2

  • 1Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7600001, Israel.

Journal of the American Chemical Society
|February 18, 2022
PubMed
概括
此摘要是机器生成的。

使用人工智能预测结构的计算酶设计使具有挑战性的多功能过氧化酶 (VP) 的功能表达成为可能. 这种进步允许对工业应用的酶家族进行更广泛的探索.

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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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科学领域:

  • 生物化学
  • 酶工程
  • 计算生物学

背景情况:

  • 白色腐烂真菌利用氧化还原酶,特别是多功能过氧化酶 (VPs),以有效地分解红素.
  • 再组合成的VP具有挑战性,限制了它们的研究和工业应用.
  • 精确的酶结构对于计算优化至关重要,但对于许多酶来说,实验结构是稀缺的.

研究的目的:

  • 评估基于深度学习的*ab initio*结构预测用于计算酶设计的可靠性.
  • 设计和功能表达具有改进性质的新型多功能过氧酶 (VPs).
  • 展示人工智能驱动的方法用于探索自然酶多样性的实用性.

主要方法:

  • 使用深度学习*ab initio*结构预测来生成VP优化模型.
  • 采用PROSS计算设计来实现VP的一次性稳定性和功能增强.
  • 在酵母中表达设计的VP变体并描述它们的活性,稳定性和反应性.

主要成果:

  • 人工智能预测的结构是计算酶设计的可靠起点.
  • 在酵母中成功表达了多达43个突变的4个设计VPs,与它们的野生类型对应物不同.
  • 三种设计在反应性和环境耐受性方面表现出显著的多样性.

结论:

  • 深度学习结构预测与计算设计相结合,可有效优化具有挑战性的酶.
  • 这种方法扩大了计算酶工程的范围,促进了新生物催化剂的发现.
  • 该方法允许从基因组数据中直接利用自然酶家族中的功能多样性.